The Gadsden flag, that includes a rattlesnake and the word "Don't Tread on Me

https://cashcizw120.trexgame.net/the-evolution-of-civil-war-flags-designs-and-meanings-2

The Gadsden flag, presenting a rattlesnake and the word "Don't Tread on Me," became followed by some Confederate units as a image of resistance in opposition t perceived oppression.